Fluorescence

Fluorescence is a luminescence, i.e. optical phenomenon in cold bodies, in which a molecule absorbs a high-energy photon, and re-emits it as a lower-energy (longer-wavelength) photon. The energy difference between the absorbed and emitted photons ends up as molecular vibrations (heat). Usually the absorbed photon is in the ultraviolet, and the emitted light (luminescence) is in the visible range, but this depends on the absorbance curve and Stokes shift of the particular fluorophore. Fluorescence is named after the mineral fluorite (calcium fluoride), which exhibits this phenomenon.

External links

• Jablonski diagram
• Fluorescence on Scienceworld
• Basic Concepts in Fluorescence
• Scorpion detection using UV LEDs
• Immunofluorescence Protocol
• Handbook on fluorescent probes used in biology from the company Molecular Probes